Application of Liquid Based Thin Layer Cell Slicing Technology

Liquid based thin layer cell section detection technology

Thin Cytological Test (TCT), a liquid based thin-layer cytological examination system, was born in 1991 in the United States and other countries. It was first applied in gynecological cytology examinations. Since 2001, research on liquid based cytology for screening cervical cancer has been carried out in China, which has rapidly developed this technology and is known as a revolution in cytological examination technology. TCT fundamentally solves the technical problems of high false negative rate, high loss rate (80%), and poor smear quality in routine exfoliative cell production, resulting in a positive detection rate of over 95% for cervical cancer, making a significant contribution to the diagnosis of exfoliative cytology. At present, it has become one of the best recommended methods for screening cervical cancer, providing a very clear diagnostic basis for early diagnosis and treatment of cervical cancer, and is a clinical testing technology that is highly worthy of promotion and application.

Kuohai Technology KCT-10 Liquid based Cell Ultrathin Automatic Slicing Machine (Membrane Type)

As early as the 1960s and 1970s, many European and American medical institutions used manual preparation of filter membranes for diagnostic cytology, and the descriptions of membrane properties were basically the same as those of current filter membranes. The several production devices and methods currently used are not particularly demanding and difficult to control, but the developers have mechanized this process. Liquid based thin-layer cell sectioning technology refers to the technology of preparing cytological specimens (smears or imprints) using an automatic thin-layer sectioning device. These devices were developed in the late 20th century and mainly include products from two American companies, namely the Thin Prep Thin Film Technology (also known as TCT) and the Auto Cell PREP Liquid Based Cell Thin Film Technology (also known as LCT). Production began in 1997 and 1998, respectively. Both TCT and LCT technologies are liquid based thin layer cell technology cytology production devices (production machines), just like pathological tissue slicing machines, which are only production machines.

However, their prices are expensive, and only some large hospitals in China can carry out this project, while the promotion in grassroots hospitals is relatively slow. Later, some domestic manufacturers developed production machines such as centrifugation and sedimentation methods, and independently developed cell preservation solutions, greatly reducing the production cost of thin layer liquid based cells, making this technology easy to accept and promote in grassroots hospitals.