Introduction to the operating principle of a fully automatic biological tissue embedding machine

The embedding machine, also known as the fully automatic biological tissue embedding machine or paraffin embedding machine, is a device that embeds tissue wax blocks in human or animal and plant specimens after dehydration and waxing, for histological diagnosis or research after slicing. It is suitable for use in medical colleges, hospital pathology departments, medical research units, animal and plant research units, and food testing departments.

Function and principle of embedding machine

Due to the different composition of various tissues in biological samples, their properties also vary, such as softness and hardness, looseness, density, area size, etc. It is almost impossible to cut them into thin slices of more than ten micrometers or even several micrometers under natural conditions. The principle of embedding is to immerse certain special supporting substances into the interior of the tissue block, utilizing the physical properties of the supporting substances, such as transforming from liquid to solid, to ensure that the entire organization has a uniform and consistent solid structure and sufficient hardness, in order to facilitate the use of slicing mechanisms to obtain extremely thin slices. The most commonly used embedding method in histological slicing technology is to use paraffin as the embedding agent, and place the tissue block soaked in paraffin to make a wax block.

Due to the softness or uneven hardness of the tissue, it is difficult to make slices with uniform thickness using conventional techniques when making slices or ultra-thin slices. Therefore, it is necessary to immerse certain substances inside the tissue, and the entire tissue will harden equally. First, melt paraffin is injected into the embedding bracket (mold), and then use tweezers to remove the tissue block that has been soaked in wax from the dehydration box, place it in the center of the embedding bracket, and place it on a small cold table, Gently press the tissue block with tweezers to achieve flatness. Cover the bottom of the dehydration box, add paraffin, and move to the freezing table. After freezing, remove the tissue wax block for cutting.

KH-BJ biological tissue embedding machine

The embedding machine is not only used to observe the morphology and structure of normal cell tissues, but also as the main method for studying, observing, and judging the morphological changes of cell tissues in disciplines such as pathology and forensic medicine. It has also been widely used in research in many other disciplines; In teaching, to achieve the observation of sliced specimens under light microscopy. Embedding solutions help you ensure correct tissue positioning, avoid thermal damage, and create ideal tissue block shapes.