Precautions for submitting pathological examination specimens

1. All tissue specimens taken during surgery should be immediately fixed in a fixative (specimen bottles and fixative should be prepared in the operating room). Generally, neutral formalin is used as the fixative. The amount of fixative should not be less than 5 times the volume of the specimen. The sample bottle mouth should be large for easy removal after fixation. If the contact between the specimen and the bottle wall or bottom affects the fixation, use a absorbent cotton pad; For those floating on the liquid surface, cover them with absorbent cotton. If there are infectious specimens, attention should be paid not to contaminate the outside of the container.

2. The specimen container should be labeled with the patient's name. If multiple tissues are taken from the same patient at the same time, or if the same tissue is taken from different parts, it should be divided into containers and marked separately.

3. When taking specimens, be careful not to use toothed forceps or forceps to clamp them, and do not compress them to avoid artificial deformation and hinder diagnosis. The specimen should not be cut open before submission, and should be kept in its original form for testing. The organization for examination should not be too small to avoid affecting diagnosis. Small (<5mm) and fragile small tissues need to be wrapped in small gauze and labeled with a pin to prevent omission.

4. If the specimen is the entire organ or a large part of it, it can be sent to the pathology department, invited by a pathologist for appropriate treatment, and then fixed for examination.

5. Samples for cytological examination of various body fluids and puncture fluids should be immediately sent for examination. If they cannot be sent for examination immediately, they should be centrifuged and made into 2-3 uniform smears. They should be immediately fixed in a mixture of ether and 95% ethanol or 95% ethanol, and then sent for examination together with the fixed solution. Vaginal discharge, nasopharyngeal or other secretions, and puncture material smears should be fixed in the above-mentioned fixative immediately after the smears are made, and then sent for examination.

6. When submitting specimens for examination, the pathological examination application form should be filled out in detail item by item, with clear handwriting and written in blue black or pure black ink. When there are special clinical requirements, they should be indicated on the application form or contacted in advance.

7. When the specimens are sent to the pathology department, they should be submitted to the recipient one by one along with the application form. If any problems are found, they should be promptly identified and signed for by the recipient.

8. When sending samples for inspection from other places or long-distance, the container should be sealed and properly packaged to avoid damage during transportation.