Application and principle of pasteurization dyeing solution

Applicable scope

Suitable for clinical cell morphology staining examination.

In gynecological examinations, Pap staining cytology is a commonly used screening method for cervical cancer and precancerous lesions. At the same time, Pap staining can also observe female hormone levels and detect infections of reproductive tract pathogens such as Candida and Trichomonas. This Pap's staining set uses EA36 staining solution, while EA50 staining solution is actually an improved version of EA36, and its operation method is similar. It is generally believed that EA36 and EA50 are more suitable for gynecological specimens, while other modified EA staining solutions are more commonly used for non gynecological specimens (such as exfoliated cells in chest and ascites).

Dyeing principle

The Pap staining solution contains dyes such as hematoxylin, orange yellow, eosin, bright green, and Bismarck brown. Among them, hematoxylin is an alkaline dye that can bind to the main chemical component deoxyribonucleic acid in the cell nucleus, resulting in a purple blue color. Orange G, eosin, and bright green are acidic dyes that can bind to proteins with opposite charges in the cytoplasm, resulting in varying degrees of red, yellow, and blue-green. The results of Pap staining show that the nucleus structure is clear, the cytoplasm is bright and bright, and the particles in the cytoplasm are distinct, making it an ideal cytological staining method.